… We thank the entire Zhang lab for their support and advice; PA Sharp for generous help with
… We thank the entire Zhang lab for their support and advice; PA Sharp for generous help with …

Recent advances in genome engineering technologies based on the CRISPR-associated
RNA-guided endonuclease Cas9 are enabling the systematic interrogation of mammalian …

Assessment and characterization of gut microbiota has become a major research area in
human disease, including type 2 diabetes, the most prevalent endocrine disease worldwide. To …

The number and diversity of known CRISPR–Cas systems have substantially increased in
recent years. Here, we provide an updated evolutionary classification of CRISPR–Cas …

… AFC also inhibits the chemotactic response of mouse peritoneal macrophages and superoxide
formation induced by the agonist f-Met-Leu-Phe in human neutrophils (120, 122, 123). In …

We report acute antibody responses to SARS-CoV-2 in 285 patients with COVID-19. Within
19 days after symptom onset, 100% of patients tested positive for antiviral immunoglobulin-G …

Soil acidification is a major problem in soils of intensive Chinese agricultural systems. We
used two nationwide surveys, paired comparisons in numerous individual sites, and several …

We report temporal patterns of viral shedding in 94 patients with laboratory-confirmed COVID-19
and modeled COVID-19 infectiousness profiles from a separate sample of 77 infector–…

Targeted genome editing technologies have enabled a broad range of research and medical
applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted to …

The Streptococcus pyogenes Cas9 (SpCas9) nuclease can be efficiently targeted to genomic
loci by means of single-guide RNAs (sgRNAs) to enable genome editing 1 , 2 , 3 , 4 , 5 , 6 , …