Applications of the Restriction Free (RF) cloning procedure for molecular manipulations and protein expression

T Unger, Y Jacobovitch, A Dantes, R Bernheim… - Journal of structural …, 2010 - Elsevier
… based on similar principles, as RF cloning. The RF cloning technology that we use is …
For the RF cloning, the gene of interest is PCR-amplified using two primers; each contains a …

Application of the Restriction-Free (RF) cloning for multicomponents assembly

Y Peleg, T Unger - DNA Cloning and Assembly Methods, 2014 - Springer
… In this chapter on the Restriction-Free (RF) methodology and … Whereas, the RF methodology
requires purification of the … of the primers for cloning using the RF and TPCR methods [12]. …

Evaluation of higher power delivery during RF pulmonary vein isolation using optimized and contiguous lesions

…, A Almorad, G Hilfiker, T Phlips, P Unger… - Journal of …, 2020 - Wiley Online Library
… the RF lesion mostly grows during the initial period of RF application. We also know that, for
different RF … In our study, the median RF time per application was significantly shorter when …

Depth profiling of electrically non-conductive layered samples by RF-GDOES and HFM plasma SNMS

VD Hodoroaba, WES Unger, H Jenett, V Hoffmann… - Applied surface …, 2001 - Elsevier
… In this work the rf-GDOES parameters varied in order to optimise the depth resolution were
the GD pressure and the rf voltage. A detailed analysis of the effect of the variation of the …

Prospective randomized evaluation of high power during CLOSE-guided pulmonary vein isolation: the POWER-AF study

…, T Phlips, M El Haddad, M Lycke, P Unger… - Circulation …, 2021 - Am Heart Assoc
… It is also known that the radiofrequency lesion mostly grows during the initial period of
radiofrequency application and that lesion size invariably increases with power. In our study, AI …

Climate forcing by the on-road transportation and power generation sectors

N Unger, DT Shindell, JS Wang - Atmospheric Environment, 2009 - Elsevier
… In a multi-pollutant approach, we apply a global atmospheric composition-climate model to
quantify the total RF from the global and United States (US) ORT and PG sectors. We assess …

Using error-source switching (ESS) concept to analyze the conducted radio frequency electromagnetic immunity of microcontrollers

T Su, M Unger, T Steinecke… - IEEE transactions on …, 2011 - ieeexplore.ieee.org
This paper introduces a new concept, the error-source switching (ESS), for the electromagnetic
immunity of microcontrollers. Under the concept of ESS, a microcontroller is a multiple-…

Radio frequency (rf) plasma‐deposited polymer films: influence of external plasma parameters as viewed by comprehensive in‐situ surface chemical analysis by XAS …

WES Unger, S Swaraj, U Oran… - Surface and Interface …, 2006 - Wiley Online Library
The formation of plasma‐deposited materials made from organic molecules is a
technologically attractive way to obtain films with unique properties, eg adhesion, wettability, …

[HTML][HTML] Impact of aviation on climate: FAA's aviation climate change research initiative (ACCRI) phase II

…, HB Selkirk, A Sokolov, N Unger… - Bulletin of the …, 2016 - journals.ametsoc.org
… (RF) estimates for individual forcing effects are provided as a range for comparison against
those published in the literature. Preliminary results for selected RF … decrease RF associated …

Analysis and Computation of a Base Labor Rate for Cost Models of Major Weapon System Acquisition

DE Knuth, RF Unger… - 1978 - apps.dtic.mil
The purpose of this thesis was to analyze and compare the Base Labor Rates determined
by the full cost approach versus the Maintenance Cost System. If the labor rates were …